Neptunomonas phycophila sp. nov. isolated from a culture of Symbiodinium sp. a dinoflagellate symbiont of the sea anemone Aiptasia tagetes.
Int J Syst Evol Microbiol. 2015 Jan 6;
Authors: Frommlet JC, Guimarães B, Sousa L, Serôdio J, Alves A
A gram staining negative, facultative anaerobic, oxidase-positive and catalase-positive, rod-shaped bacterium strain SYM1T was isolated from a ITS2-type B1 culture of Symbiodinium spp., an algal symbiont of the sea anemone Aiptasia tagetes collected in Puerto Rico. Growth was observed at 4-40 °C (optimum 30 °C), at pH 5.0-11.0 (optimum pH 8.0) and with 0.5-8% (optimum 2%) NaCl (w/v). Phylogenetic analyses of 16S rRNA gene sequences showed that strain SYM1T was a member of the genus Neptunomonas with the type strain of Neptunomonas naphthovorans as the closest phylogenetic relative with a pairwise sequence similarity of 98.15%. However, DNA-DNA relatedness between SYM1T and N. naphthovorans CIP 106451T was 24%. Moreover, SYM1T could be distinguished from its closest relative by several phenotypic characteristics such as NaCl, pH and temperature tolerance, nitrate reduction and utilization of carbon substrates. The major cellular fatty acids were C16:0, C18:1ω7c and summed feature 3 (comprising C16:1ω7c and/or iso-C15:0 2-OH). Genomic DNA G+C content of strain SYM1T was 45 mol%. Q-8 was the only respiratory quinone detected. Based on a polyphasic taxonomic characterization, strain SYM1T represents a novel species in the genus Neptunomonas for which the name Neptunomonas phycophila sp. nov. is proposed. The type strain is SYM1T (= LMG 28329T = CECT 8716T).
PMID: 25563909 [PubMed - as supplied by publisher]