November 2020 archive

EukRef-excavates: seven curated SSU ribosomal RNA gene databases.

Related Articles EukRef-excavates: seven curated SSU ribosomal RNA gene databases. Database (Oxford). 2020 Nov 20;2020: Authors: Kolisko M, Flegontova O, Karnkowska A, Lax G, Maritz JM, Pánek T, Táborský P, Carlton JM, Čepička I, Horák A, Lukeš J, Simpson AGB, Tai V Abstract The small subunit ribosomal RNA (SSU rRNA) gene is a widely used …

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Evolutionary history of mitochondrial genomes in Discoba, including the extreme halophile Pleurostomum flabellatum (Heterolobosea).

Related Articles Evolutionary history of mitochondrial genomes in Discoba, including the extreme halophile Pleurostomum flabellatum (Heterolobosea). Genome Biol Evol. 2020 Nov 13;: Authors: Ettahi K, Lhee DH, Sung JY, Simpson AGB, Park JS, Yoon HS Abstract Data from Discoba (Heterolobosea, Euglenozoa, Tsukubamonadida, and Jakobida) are essential to understand the evolution of mitochondrial genomes (mitogenomes), since …

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Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates.

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Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates.
Talanta. 2021 Jan 15;222:121416
Authors: Morais SL, Barros P, Santos M, Delerue-Matos C, Gomes AC, Fátima Barroso…

Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates

This work addresses the development of a disposable electrochemical genosensor for the detection of the toxic dinoflagellate, Alexandrium minutum. Analyzing public databases, a specific 70 bp DNA probe, targeting A. minutum, was selected and designed. The genosensor methodology implied the immobilization of a A. minutum-specific DNA-capture probe onto screen-printed gold electrodes (SPGE). To improve both the selectivity and to avoid strong secondary structures, that could hinder the…

A New Dinoflagellate Genome Illuminates a Conserved Gene Cluster Involved in Sunscreen Biosynthesis.

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A New Dinoflagellate Genome Illuminates a Conserved Gene Cluster Involved in Sunscreen Biosynthesis.
Genome Biol Evol. 2020 Nov 04;:
Authors: Shoguchi E, Beedessee G, Hisata K, Tada I, Narisoko H, S…

Food or just a free ride? A meta-analysis reveals the global diversity of the Plastisphere.

Related Articles Food or just a free ride? A meta-analysis reveals the global diversity of the Plastisphere. ISME J. 2020 Nov 02;: Authors: Wright RJ, Langille MGI, Walker TR Abstract It is now indisputable that plastics are ubiquitous and problematic in ecosystems globally. Many suggestions have been made about the role that biofilms colonizing plastics …

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A New Dinoflagellate Genome Illuminates a Conserved Gene Cluster Involved in Sunscreen Biosynthesis

Photosynthetic dinoflagellates of the Family Symbiodiniaceae live symbiotically with many organisms that inhabit coral reefs and are currently classified into fifteen groups, including seven genera. Draft genomes from four genera, Symbiodinium, Breviolum, Fugacium, and Cladocopium, which have been isolated from corals, have been reported. However, no genome is available from the genus Durusdinium, which occupies an intermediate phylogenetic position in the Family Symbiodiniaceae and is well…

The 5S rRNA genes in Alexandrium: their use as a FISH chromosomal marker in studies of the diversity, cell cycle and sexuality of dinoflagellates.

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The 5S rRNA genes in Alexandrium: their use as a FISH chromosomal marker in studies of the diversity, cell cycle and sexuality of dinoflagellates.
Harmful Algae. 2020 Sep;98:101903
Authors: de Busto…

The 5S rRNA genes in Alexandrium: their use as a FISH chromosomal marker in studies of the diversity, cell cycle and sexuality of dinoflagellates

Chromosomal markers of the diversity and evolution of dinoflagellates are scarce because the genomes of these organisms are unique among eukaryotes in terms of their base composition and chromosomal structure. Similarly, a lack of appropriate tools has hindered studies of the chromosomal localization of 5S ribosomal DNA (rDNA) in the nucleosome-less chromosomes of dinoflagellates. In this study, we isolated and cloned 5S rDNA sequences from various toxin-producing species of the genus…