Category: Dinoflagellate genomics

The Hidden Sexuality of Alexandrium Minutum: An Example of Overlooked Sex in Dinoflagellates.
PLoS One. 2015;10(11):e0142667
Authors: Figueroa RI, Dapena C, Bravo I, Cuadrado A
Abstract
Dinoflagella…

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Multifunctional polyketide synthase genes identified by genomic survey of the symbiotic dinoflagellate, Symbiodinium minutum.
BMC Genomics. 2015;16(1):941
Authors: Beedessee G, Hisata K, Roy MC, Sat…

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RNA Sequencing Revealed Numerous Polyketide Synthase Genes in the Harmful Dinoflagellate Karenia mikimotoi.
PLoS One. 2015;10(11):e0142731
Authors: Kimura K, Okuda S, Nakayama K, Shikata T, Takahash…

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The Symbiodinium kawagutii genome illuminates dinoflagellate gene expression and coral symbiosis.
Science. 2015 Nov 6;350(6261):691-4
Authors: Lin S, Cheng S, Song B, Zhong X, Lin X, Li W, Li L, Zha…

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Corrigendum to “Heat Shock Protein 70 and 90 Genes in the Harmful Dinoflagellate Cochlodinium polykrikoides: Genomic Structures and Transcriptional Responses to Environmental Stresses”.
Int J Genomics. 2015…

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Seasonal Preservation Success of the Marine Dinoflagellate Coral Symbiont, Symbiodinium sp.
PLoS One. 2015;10(9):e0136358
Authors: Hagedorn M, Carter VL
Abstract
Coral reefs are som…

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Identification of microRNAs in the Toxigenic Dinoflagellate Alexandrium catenella by High-Throughput Illumina Sequencing and Bioinformatic Analysis.
PLoS One. 2015;10(9):e0138709
Authors: Geng H, S…

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The genome of Aiptasia, a sea anemone model for coral symbiosis.
Proc Natl Acad Sci U S A. 2015 Aug 31;
Authors: Baumgarten S, Simakov O, Esherick LY, Liew YJ, Lehnert EM, Michell CT, Li Y, Hambleto…

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Paralytic shellfish toxin biosynthesis in cyanobacteria and dinoflagellates: a molecular overview.
J Proteomics. 2015 Aug 24;
Authors: Wang DZ, Zhang SF, Zhang Y, Lin L
Abstract
Para…

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Heterologous DNA Uptake in Cultured Symbiodinium spp. Aided by Agrobacterium tumefaciens.

PLoS One. 2015;10(7):e0132693

Authors: Ortiz-Matamoros MF, Islas-Flores T, Voigt B, Menzel D, Baluška F, Villanueva MA

Abstract
Plant-targeted pCB302 plasmids containing sequences encoding gfp fusions with a microtubule-binding domain; gfp with the fimbrin actin-binding domain 2; and gfp with AtRACK1C from Arabidopsis thaliana, all harbored in Agrobacterium tumefaciens, were used to assay heterologous expression on three different clades of the photosynthetic dinoflagellate, Symbiodinium. Accessibility to the resistant cell wall and through the plasma membrane of these dinoflagellates was gained after brief but vigorous shaking in the presence of glass beads and polyethylene glycol. A resistance gene to the herbicide Basta allowed appropriate selection of the cells expressing the hybrid proteins, which showed a characteristic green fluorescence, although they appeared to lose their photosynthetic pigments and did not further divide. Cell GFP expression frequency measured as green fluorescence emission yielded 839 per every 106 cells for Symbiodinium kawagutii, followed by 640 and 460 per every 106 cells for Symbiodinium microadriaticum and Symbiodinium sp. Mf11, respectively. Genomic PCR with specific primers amplified the AtRACK1C and gfp sequences after selection in all clades, thus revealing their presence in the cells. RT-PCR from RNA of S. kawagutii co-incubated with A. tumefaciens harboring each of the three vectors with their respective constructs, amplified products corresponding to the heterologous gfp sequence while no products were obtained from three distinct negative controls. The reported procedure shows that mild abrasion followed by co-incubation with A. tumefaciens harboring heterologous plasmids with CaMV35S and nos promoters can lead to expression of the encoded proteins into the Symbiodinium cells in culture. Despite the obvious drawbacks of the procedure, this is an important first step towards a stable transformation of Symbiodinium.

PMID: 26167858 [PubMed – in process]